Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- PAB0742 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB0742, RRID:AB_1112905
- Product name
- ATG7 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of ATG7.
- Storage
- Store at 4°C on dry atmosphere.After reconstitution with deionized water, store at -20°C or lower.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot on Peripheral Blood Mononuclear Cells (PBMCs) lysate using rabbit ATG7 polyclonal antibody (Cat # PAB0742) at a dilution of 1 : 100 (ECL) .
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Confocal microscopy on cytospin - isolated human blood cells. A : Neutrophils seem to be stained. B : The cells were double - stained for ATG7 appearing in red and Myeloperoxidase (MPO) appearing in green. Using rabbit ATG7 polyclonal antibody (Cat # PAB0742) at a dilution of 1 : 100, incubated for 1 hr at room temperature. The cells stained for ATG7 appear in red. The cells were counter stained with Hoechst Dye (blue colour) . Here, the merged picture is presented. Observations : No staining was observed using the Pre - immunisation serum. No staining was observed in HL-60 cell line.
- Validation comment
- Immunofluorescence