F54380

antibody from NSJ Bioreagents

Targeting: ATG7 APG7L, DKFZp434N0735, GSA7

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

F54380

Provider

NSJ Bioreagents

Product name

ATG7 Antibody / APG7

Antibody type

Polyclonal

Description

Antigen affinity purified

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Vial size

0.08 µl, 0.4 µl

Storage

Aliquot the ATG7 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) human HepG2 and 2) mouse NIH 3T3 cell lysate with ATG7 antibody. Predicted molecular weight: 70-80 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) Caki-1, 2) HeLa and 3) HepG2 cell lysate with ATG7 antibody. Predicted molecular weight: 70-80 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human HEK293 cell lysate with ATG7 antibody. Predicted molecular weight: 70-80 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of treated and untreated human U-251 cells with ATG7 antibody (green) and DAPI nuclear stain (blue).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human liver tissue with ATG7 antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.