HPA013189

TCF21 antibody from Atlas Antibodies

bHLHa23, POD1

Eligible for validation within the Antibodypedia Validation Initiative

Western blot

Supportive data in Antibodypedia

Immunohistochemistry

Supportive data in Antibodypedia

Provider product page for HPA013189

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Antibody Data

Product number

HPA013189

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA013189, RRID:AB_10601215

Product name

Anti-TCF21

Provider product page

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

LSDVEDLQEVEMLECDGLKMDSNKEFVTSNESTEE
SSNCENGSPQKGRGGLGKRRKAPTKKSPLSGVSQE
GKQV


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References

Characterization of TCF21 Downstream Target Regions Identifies a Transcriptional Network Linking Multiple Independent Coronary Artery Disease Loci
Sazonova O, Zhao Y, Nürnberg S, Miller C, Pjanic M, Castano V, Kim J, Salfati E, Kundaje A, Bejerano G, Assimes T, Yang X, Quertermous T, McCarthy M
PLOS Genetics 2015 May;11(5)

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Coronary Artery Disease Associated Transcription Factor TCF21 Regulates Smooth Muscle Precursor Cells That Contribute to the Fibrous Cap
Nurnberg S, Cheng K, Raiesdana A, Kundu R, Miller C, Kim J, Arora K, Carcamo-Oribe I, Xiong Y, Tellakula N, Nanda V, Murthy N, Boisvert W, Hedin U, Perisic L, Aldi S, Maegdefessel L, Pjanic M, Owens G, Tallquist M, Quertermous T, McCarthy M
PLOS Genetics 2015 May;11(5)

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in control (vector only transfected HEK293T lysate) and tCF21 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY418825).

Sample type

HUMAN

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human colon shows moderate cytoplasmic and nuclear positivity in glandular cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human endometrium shows moderate nuclear positivity in a subset of glandular cells.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex shows no nuclear positivity in either neurons or glial cells as expected.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human kidney shows moderate nuclear positivity in cells in tubules.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human lung shows strong nuclear positivity in a subset of pneumocytes.

Sample type

HUMAN