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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [4]
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Validation data
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- Product number
- PA5-53031 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-TCF21 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: LSDVEDLQEV EMLECDGLKM DSNKEFVTSN ESTEESSNCE NGSPQKGRGG LGKRRKAPTK KSPLSGVSQE GKQV Highest antigen sequence identity to the following orthologs: Mouse - 93%, Rat - 93%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.20 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TCF21 in control (vector only transfected HEK293T lysate) and tCF21 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a TCF21 Polyclonal Antibody (Product # PA5-53031).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of TCF21 in human kidney using a TCF21 Polyclonal Antibody (Product # PA5-53031) shows moderate nuclear positivity in a subset of tubules as well as in some glomerular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of TCF21 in human lung using a TCF21 Polyclonal Antibody (Product # PA5-53031) shows strong nuclear positivity in a subset of pneumocytes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of TCF21 in human endometrium using a TCF21 Polyclonal Antibody (Product # PA5-53031) shows moderate nuclear positivity in a subset of glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of TCF21 in human cerebral cortex using a TCF21 Polyclonal Antibody (Product # PA5-53031) shows no nuclear positivity in either neurons or glial cells as expected.
