H00006446-M01

antibody from Abnova Corporation

Targeting: SGK1 SGK

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00006446-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00006446-M01, RRID:AB_425681

Product name

SGK monoclonal antibody (M01), clone 4D7-G3

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant SGK.

Antigen sequence

MTVKTEAAKGTLTYSRMRGMVAILIAFMKQRRMGL
NDFIQKIANNSYACKHPEVQSILKISQPQEPELMN
ANPSPPPSPSQQINLGPSSNPHAKPSDFHFLKVIG
KGSFGKVLLARHKAEEVFYAVKVLQKKAILKKKEE
KHIMSERNVLLKNVKHPFLVGLHFSFQTADKLYFV
LDYINGGELFYHLQRERCFLEPRARFYAAEIASAL
GYLHSLNIVYRDLKPENILLDSQGHIVLTDFGLCK
ENIEHNSTTSTFCGTPEYLAPEVLHKQPYDRTVDW
WCLGAVLYEMLYGLPPFYSRNTAEMYDNILNKPLQ
LKPNITNSARHLLEGLLQKDRTKRLGAKDDFMEIK
SHVFFSLINWDDLINKKITPPFNPNVSGPNDLRHF
DPEFTEEPVPNSIGKSPDSVLVTASVKEAAEAFLG
FSYAPPTDSFL


Isotype

IgG

Antibody clone number

4D7-G3

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of SGK expression in transfected 293T cell line by SGK monoclonal antibody (M01), clone 4D7-G3.Lane 1: SGK transfected lysate(48.9 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged SGK is approximately 1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to SGK on HeLa cell. [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to SGK on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to SGK on formalin-fixed paraffin-embedded human stomach tissue. [antibody concentration 5 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol