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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- H00006446-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00006446-M01, RRID:AB_548058
- Product name
- Mouse Monoclonal SGK1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. SGK - serum/glucocorticoid regulated kinase
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SGK Antibody (4D7-G3) [H00006446-M01] - Analysis of SGK over-expressed 293 cell line, cotransfected with SGK Validated Chimera RNAi ( Cat # H00006446-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with SGK monoclonal antibody (M01) clone 4D7-G3 (Cat # H00006446-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SGK Antibody (4D7-G3) [H00006446-M01] - Western Blot analysis of SGK expression in A-549 ( Cat # L025V1 ).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SGK Antibody (4D7-G3) [H00006446-M01] - Analysis of SGK expression in transfected 293T cell line by SGK monoclonal antibody (M01), clone 4D7-G3.Lane 1: SGK transfected lysate(48.9 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: SGK1 Antibody (4D7-G3) [H00006446-M01] - SGK Antibody (4D7-G3) [H00006446-M01] - Detection limit for recombinant GST tagged SGK is approximately 1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: SGK Antibody (4D7-G3) [H00006446-M01] - Analysis of monoclonal antibody to SGK on HeLa cell. Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SGK Antibody (4D7-G3) [H00006446-M01] - Analysis of monoclonal antibody to SGK on formalin-fixed paraffin-embedded human stomach tissue. Antibody concentration 5 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SGK Antibody (4D7-G3) [H00006446-M01] - Analysis of monoclonal antibody to SGK on formalin-fixed paraffin-embedded human kidney. Antibody concentration 3 ug/ml.
