21602-1-AP

antibody from Invitrogen Antibodies

Targeting: ALDH8A1 ALDH12

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

21602-1-AP

Provider

Invitrogen Antibodies

Product name

ALDH8A1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: CMHYTVRAP VGVAGLISPW NLPLYLLTWK IAPAMAAGNT VIAKPSELTS VTAWMLCKLL DKAGVPPGVV NIVFGTGPRV GEALVSHPEV PLISFTGSQP TAERITQLSA PHCKKLSLEL GGKNPAIIFE DANLDECIPA TVRSSFANQG EICLCTSRIF VQKSIYSEFL KRFVEATRKW KVGIPSDPLV SIGALISKAH LEKVRSYVKR ALAEGAQIWC GEGVDKLSLP ARNQAGYFML PTVITDIKDE SCCMTEEIFG PVTCVVPFDS EEEVIERANN VKYGLAATVW SSNVGRVHRV AKKLQSGLVW TNCWLIRELN LPFGGMKSSG IGREGAKDSY DFFTEIKTIT VKH (136-487 aa encoded by BC113862)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.19 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

L02 cells were subjected to SDS PAGE followed by western blot with 21602-1-AP (ALDH8A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

L02 cells were subjected to SDS PAGE followed by western blot with 21602-1-AP (ALDH8A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Various lysates were subjected to SDS PAGE followed by western blot with 21602-1-AP (ALDH8A1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21602-1-AP (ALDH8A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( -20°C Ethanol ) fixed HepG2 cells using 21602-1-AP (ALDH8A1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( -20°C Ethanol ) fixed HepG2 cells using 21602-1-AP (ALDH8A1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human kidney using 21602-1-AP (ALDH8A1 antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human kidney using 21602-1-AP (ALDH8A1 antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human hepatocirrhosis using 21602-1-AP (ALDH8A1 antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human hepatocirrhosis using 21602-1-AP (ALDH8A1 antibody) at dilution of 1:50 (under 40x lens).