Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- APR-067-25UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Prostaglandin F2- alpha Receptor/PTGFR Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Reconstitution: 1 X 50 µL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 25 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) and human U-87 MG glioblastoma cell line (lanes 3 and 6)lysates: - 1-3. Anti-Prostaglandin F2- alpha Receptor/PTGFR Antibody (#APR-067), (1:200). 4-6. Anti-Prostaglandin F2- alpha Receptor/PTGFR Antibody , preincubated with Prostaglandin F2- alpha Receptor/PTGFR Blocking Peptide (#BLP-PR067).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of Prostaglandin F2-alpha receptor in mouse hippocampus - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Prostaglandin F2- alpha Receptor/PTGFR Antibody (#APR-067) (1:200), followed by goat Anti-rabbit-AlexaFluor-488. PTGFR staining (green) in mouse hippocampal dentate gyrus is detected in cell bodies (arrows). Cell nuclei are stained with DAPI (blue). Mouse brain hippocampal dentate gyrus sections were obtained two hours following kainate acidintraventricular injection.