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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
- Other assay [1]
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Validation data
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- Product number
- A302-981A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- eIF4AIII/EIF4A3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.20 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse eIF4AIII/EIF4A3 by western blot. Samples: Whole cell lysate from HeLa (5, 15, and 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibody: Affinity purified rabbit anti-eIF4AIII/EIF4A3 antibody (Product # A302-981A; lot A302-981A-1) used at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse eIF4AIII/EIF4A3 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and mouse NIH3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-eIF4AIII/EIF4A3 antibody A302-981A used for WB at 0.04 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 3 µg/mg lysate. eIF4AIII/EIF4A3 was also immunoprecipitated by rabbit anti-eIF4AIII/EIF4A3 antibody A302-980A, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human eIF4AIII/EIF4A3 by immunohistochemistry. Samples: FFPE serial sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- eIF4AIII/EIF4A3 (Cat. No. A302-981A Lot1 left panel and Cat. No. A302-980A Lot1 right panel) used at a dilution of 1:200 (1µg/ml) and 1:5,000 (0.2µg/ml). Detection: DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of mouse eIF4AIII/EIF4A3 by immunohistochemistry. Sample: FFPE section of mouse renal carcinoma. Antibody: Affinity purified rabbit anti- eIF4AIII/EIF4A3 (Cat. No. A302-981A Lot1) used at a dilution of 1:200 (1µg/ml). Detection: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human eIF4AIII/EIF4A3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-eIF4AIII/EIF4A3 antibody (Product # A302-981A; lot A302-981A-1) used for IP at 3 µg/mg lysate. eIF4AIII/EIF4A3 was also immunoprecipitated y rabbit anti-eIF4AIII/EIF4A3 antibody (Product # A302-980A), which recognizes a downstream epitope. For blotting immunoprecipitated eIF4AIII/EIF4A3 (Product # A303-928A) was used at 0.4 µg/mL. Detection: Chemiluminescence with exposure time of 30 seconds.
