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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- NB100-93322 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-93322, RRID:AB_1237255
- Product name
- Rabbit Polyclonal Nup53 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Localization of Pom121 to the inner nuclear membrane is required for an early step of interphase nuclear pore complex assembly.
Funakoshi T, Clever M, Watanabe A, Imamoto N
Molecular biology of the cell 2011 Apr;22(7):1058-69
Molecular biology of the cell 2011 Apr;22(7):1058-69
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Nup53 Antibody [NB100-93322] - Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NUP35 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Nup53 Antibody [NB100-93322] - Detection of human NUP35 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NUP35 antibody NB100-93322 (lot NB100-93322-2) used for IP at 6 ug per reaction. NUP35 was also immunoprecipitated by a previous lot of this antibody (lot NB100-93322-1) and another rabbit anti-NUP35 antibody. For blotting immunoprecipitated NUP35, NB100-93322 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Nup53 Antibody [NB100-93322] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- NUP35 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
