Explore
Validate
LearnH00025984-M01
antibody from Abnova Corporation
Targeting: KRT23
CK23, DKFZP434G032, HAIK1, K23, MGC26158
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- H00025984-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00025984-M01, RRID:AB_425921
- Product name
- KRT23 monoclonal antibody (M01), clone 2B4-3B5
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant KRT23.
- Antigen sequence
MNSGHSFSQTPSASFHGAGGGWGRPRSFPRAPTVH
GGAGGARISLSFTTRSCPPPGGSWGSGRSSPLLGG
NGKATMQNLNDRLASYVEKVRALEEANMKLESRIL
KWHQQRDPGSKKDYSQYEENITHLQEQIVDGKMTN
AQIILLIDNARMAVDDFNLKYENEHSFKKDLEIEV
EGLRRTLDNLTIVTTDLEQEVEGMRKELILMKKHH
EQEMEKHHVPSDFNVNVKVDTGPREDLIKVLEDMR
QEYELIIKKKHRDLDTWYKEQSAAMSQEAASPATV
QSRQGDIHELKRTFQALEIDLQTQYSTKSALENML
SETQSRYSCKLQDMQEIISHYEEELTQLRHELERQ
NNEYQVLLGIKTHLEKEITTYRRLLEGESEGTREE
SKSSMKVFATPKIKAITQETINGRLVLCQVNEIQK
HA- Isotype
- IgG
- Antibody clone number
- 2B4-3B5
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Markers of progression in early-stage invasive breast cancer: a predictive immunohistochemical panel algorithm for distant recurrence risk stratification.
Aleskandarany MA, Soria D, Green AR, Nolan C, Diez-Rodriguez M, Ellis IO, Rakha EA
Breast cancer research and treatment 2015 Jun;151(2):325-33
Breast cancer research and treatment 2015 Jun;151(2):325-33
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- KRT23 monoclonal antibody (M01), clone 2B4-3B5 Western Blot analysis of KRT23 expression in Hela S3 NE ( Cat # L013V3 ).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged KRT23 is 3 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to KRT23 on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to KRT23 on formalin-fixed paraffin-embedded human ovarian cancer. [antibody concentration 5 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
