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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
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Validation data
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- Product number
- R31258 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Collagen Type 1 Antibody
- Antibody type
- Polyclonal
- Antigen
- An amino acid sequence from the C-terminus of mouse COL1A1 (QPPQEKSQDGGRYYRA) was used as the immunogen for this Collagen type 1 antibody (100% rat homology).
- Description
- Antigen affinity purified antibody
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 µg
- Concentration
- Lyophilized; resuspend with 200 ul for 0.5 mg/ml
- Storage
- After reconstitution, the Collagen type 1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of Collagen type 1 antibody and Lane 1: rat lung; 2: human placenta; 3: rat testis tissue lysate. Expected/observed size ~130KD
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ICC testing of Collagen type 1 antibody and NIH3T3 cells
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: Collagen type 1 antibody testing of human intestine cancer tissue
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-F testing of Collagen type 1 antibody and human placenta tissue
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: Collagen type 1 antibody testing of rat lung tissue
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: Collagen 1 antibody testing of mouse lung tissue at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
