ABR-004-50UL

antibody from Invitrogen Antibodies

Targeting: NMBR BB1, BB1R

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

ABR-004-50UL

Provider

Invitrogen Antibodies

Product name

Bombesin Receptor 1 (NMBR) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

50 µL

Concentration

0.6 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates: - 1,2. Anti-Bombesin Receptor 1 (NMBR) Antibody (#ABR-004), (1:1000).3,4. Anti-Bombesin Receptor 1 (NMBR) Antibody , preincubated with Bombesin Receptor 1/NMBR Blocking Peptide (#BLP-BR004).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of human lung carcinoma NCI-H526 (lanes 1 and 3) and human prostate carcinoma PC-3 (lanes 2 and 4) cell line lysates: - 1,2. Anti-Bombesin Receptor 1 (NMBR) Antibody (#ABR-004), (1:1000).3,4. Anti-Bombesin Receptor 1 (NMBR) Antibody , preincubated with Bombesin Receptor 1/NMBR Blocking Peptide (#BLP-BR004).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates: - 1,2. Anti-Bombesin Receptor 1 (NMBR) Antibody (#ABR-004), (1:1000).3,4. Anti-Bombesin Receptor 1 (NMBR) Antibody , preincubated with Bombesin Receptor 1/NMBR Blocking Peptide (#BLP-BR004).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Expression of Bombesin receptor 1 in a human breast cancer cell line - Immunocytochemical staining of paraformaldehyde-fixed and permeabilized mammary gland adenocarcinoma MDA-MB-231.A. Cells were stained with Anti-Bombesin Receptor 1 (NMBR) Antibody (#ABR-004), (1:1000), followed by goat Anti-rabbit-AlexaFluor-555 secondary Antibody (red).Hoechst 33342 (blue) is used to visualize the nuclei. B. Live view of the same field as in (A).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Expression of Bombesin receptor 1 in rat hypothalamus - Immunohistochemical staining of frozen rat hypothalamus sections using Anti-Bombesin Receptor 1 (NMBR) Antibody (#ABR-004), (1:200). A. Staining (red) appears in the neuropil near the ventricle (asterisk) and in neurons (triangles).Calbindin D28k staining (green) appears in neurons. C.Merge of (A) and (B) shows co-expression of BB1R and Calbindin in a few neurons (vertical arrow). DAPI is used as the counterstain.