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Western blot
Immunocytochemistry
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- 668501 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#668501, RRID:AB_2564439
- Product name
- Purified anti-TFIIB
- Antibody type
- Monoclonal
- Antigen
- Recombinant protein.
- Description
- TFIIB is one of the ubiquitous transcription factors required for transcription initiation by RNA polymerase II. It plays an important role in pre-initiation complex assembly, which provides a bridge between promoter-bound TFIID and RNA polymerase II. Moreover, TFIIB also directly modulates the catalytic center of RNA polymerase II.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Antibody clone number
- IIB1
- Vial size
- 100 µg
- Concentration
- 1.0 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Western blot analysis of HeLa (lane 1), Jurkat (lane 2), and Raw264.7 (lane 3). Anti-TFIIB antibody (clone IIB1) was used, followed by HRP anti-mouse IgG antibody. β-actin antibody was used as a loading control.
- Conjugate
- Unconjugated
