Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- MA5-31853 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PLAGL1 Monoclonal Antibody (8D8C5)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 8D8C5
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references HAND1 and BARX1 Act as Transcriptional and Anatomic Determinants of Malignancy in Gastrointestinal Stromal Tumor.
Hemming ML, Coy S, Lin JR, Andersen JL, Przybyl J, Mazzola E, Abdelhamid Ahmed AH, van de Rijn M, Sorger PK, Armstrong SA, Demetri GD, Santagata S
Clinical cancer research : an official journal of the American Association for Cancer Research 2021 Mar 15;27(6):1706-1719
Clinical cancer research : an official journal of the American Association for Cancer Research 2021 Mar 15;27(6):1706-1719
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PLAGL1 in HEK293 (1) and PLAGL1 cell lysate. Samples were incubated with PLAGL1 monoclonal antibody (Product # MA5-31853) using a dilution of 1:500-1:2000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PLAGL1 in human PLAGL1 recombinant protein. Sample was incubated with PLAGL1 monoclonal antibody (Product # MA5-31853) using a dilution of 1:500-1:2000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ELISA analysis of PLAGL1 in Control Antigen (black line, 100 ng); Antigen (purple line, 10 ng); Antigen (blue line, 50 ng); Antigen (red line, 100 ng). Samples were incubated with PLAGL1 monoclonal antibody (Product # MA5-31853) using a dilution of 1:10,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of PLAGL1 in paraffin-embedded ovarian cancer tissue. Sample was incubated with PLAGL1 monoclonal antibody (Product # MA5-31853) using a dilution of 1:200-1:1000 followed by DAB staining.