PAB20374

antibody from Abnova Corporation

Targeting: GPSM2 DFNB82, LGN, Pins

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB20374

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB20374, RRID:AB_10963672

Product name

GPSM2 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant GPSM2.

Antigen sequence

REVGDKSGELTARLNLSDLQMVLGLSYSTNNSIMS
ENTEIDSSLNGVRPKLGRRHSMENMELMKLTPEKV
QNWNSEILAKQKPLIAKPSAKLLFVNRLKGKKYKT
NSSTKVLQDASNSIDHRIPNSQRKISADTIGDEGF
FDL


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells with GPSM2 polyclonal antibody (Cat # PAB20374) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251 MG with GPSM2 polyclonal antibody (Cat # PAB20374) at 1-4 ug/mL dilution shows positivity in cytoplasm.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebellum with GPSM2 polyclonal antibody (Cat # PAB20374) shows strong cytoplasmic positivity in Purkinje cells in molecular layer and granular layer at 1:500-1:1000 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)