R1503

antibody from OriGene

Targeting: ANAPC11 APC11, Apc11p, HSPC214, MGC882

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

R1503

Provider

OriGene

Product name

Apc11 (ANAPC11) rabbit polyclonal antibody, Serum

Antibody type

Polyclonal

Description

Apc11 (ANAPC11) rabbit polyclonal antibody, Serum

Host

Rabbit

Conjugate

Unconjugated

Epitope

ANAPC11

Antibody clone number

NULL

Vial size

100 µl

Concentration

85 mg/ml (by Refractometry)

Other assay

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Figure 1. Conjugation pathways for ubiquitin and ubiquitin-like modifiers (UBLs). Most modifiers mature by proteolytic processing from inactive precursors (a; amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the mdifiers. Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein) /CBC(cullin-2/elongin B/elongin C)-like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked headto-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin. Data contributed by S.Jentsch, see reference 3.

Validation comment

Assay