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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AP6246a - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AP6246a, RRID:AB_2188946
- Product name
- SIRT7 Antibody (C-term)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 0.35 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Submitted references STAT3-dependent effects of IL-22 in human keratinocytes are counterregulated by sirtuin 1 through a direct inhibition of STAT3 acetylation.
Sirtuin-3 (SIRT3), a novel potential therapeutic target for oral cancer.
Sestito R, Madonna S, Scarponi C, Cianfarani F, Failla CM, Cavani A, Girolomoni G, Albanesi C
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2011 Mar;25(3):916-27
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2011 Mar;25(3):916-27
Sirtuin-3 (SIRT3), a novel potential therapeutic target for oral cancer.
Alhazzazi TY, Kamarajan P, Joo N, Huang JY, Verdin E, D'Silva NJ, Kapila YL
Cancer 2011 Apr 15;117(8):1670-8
Cancer 2011 Apr 15;117(8):1670-8
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Western blot analysis of SIRT7 (arrow) using SIRT7 Antibody (C-term) (Cat. #AP6246a). HEK293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SIRT7 gene (Lane 2).
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma."
- Primary Ab dilution
- 1:50~100
