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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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- Product number
- H00051547-D01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00051547-D01, RRID:AB_10628671
- Product name
- SIRT7 MaxPab rabbit polyclonal antibody (D01)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against a full-length human SIRT7 protein.
- Antigen sequence
MAAGGLSRSERKAAERVRRLREEQQRERLRQVSRI
LRKAAAERSAEEGRLLAESADLVTELQGRSRRREG
LKRRQEEVCDDPEELRGKVRELASAVRNAKYLVVY
TGAGISTAASIPDYRGPNGVWTLLQKGRSVSAADL
SEAEPTLTHMSITRLHEQKLVQHVVSQNCDGLHLR
SGLPRTAISELHGNMYIEVCTSCVPNREYVRVFDV
TERTALHRHQTGRTCHKCGTQLRDTIVHFGERGTL
GQPLNWEAATEAASRADTILCLGSSLKVLKKYPRL
WCMTKPPSRRPKLYIVNLQWTPKDDWAALKLHGKC
DDVMRLLMAELGLEIPAYSRWQDPIFSLATPLRAG
EEGSHSRKSLCRSREEAPPGDRGAPLSSAPILGGW
FGRGCTKRTKRKKVT- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Dicer interacts with SIRT7 and regulates H3K18 deacetylation in response to DNA damaging agents.
Zhang PY, Li G, Deng ZJ, Liu LY, Chen L, Tang JZ, Wang YQ, Cao ST, Fang YX, Wen F, Xu Y, Chen X, Shi KQ, Li WF, Xie C, Tang KF
Nucleic acids research 2016 May 5;44(8):3629-42
Nucleic acids research 2016 May 5;44(8):3629-42
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- SIRT7 MaxPab rabbit polyclonal antibody. Western Blot analysis of SIRT7 expression in mouse liver.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of SIRT7 expression in transfected 293T cell line (H00051547-T01) by SIRT7 MaxPab polyclonal antibody.Lane 1: SIRT7 transfected lysate(44.90 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of purified MaxPab antibody to SIRT7 on HeLa cell. [antibody concentration 30 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of SIRT7 transfected lysate using anti-SIRT7 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with SIRT7 purified MaxPab mouse polyclonal antibody (B01P) (H00051547-B01P).
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
