PA5-87543

antibody from Invitrogen Antibodies

Targeting: SIRT7

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-87543

Provider

Invitrogen Antibodies

Product name

SIRT7 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: NREYVRVFDV TERTALHRHQ TGRTCHKCGT QLRDTIVHFG ERGTLGQPLN WEAATEAASR ADTILCLGSS LKVLKKYPRL WCMTKPPSRR PKLYIVNLQW TPKDDWAALK LHGKCDDVMR LLMAELGLEI PAYSRWQDPI FSLATPLRAG EEGSHSRKSL CRSREEAPPG DRGAPLSSAP ILGGWFGRGC TKRTKRKKVT; Positive Samples: HepG2, SW480; Cellular Location: Cytoplasm, Nucleus, nucleolus

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.59 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Sirtuin 7 Regulates Nitric Oxide Production and Apoptosis to Promote Mycobacterial Clearance in Macrophages.

Zhang S, Liu Y, Zhou X, Ou M, Xiao G, Li F, Wang Z, Wang Z, Liu L, Zhang G
Frontiers in immunology 2021;12:779235

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of extracts of various cell lines, using SIRT7 Polyclonal antibody (Product # PA5-87543) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 90s.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of SIRT7 in extracts of various cell lines using SIRT7 Polyclonal Antibody (Product # PA5-87543) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of HeLa cells using SIRT7 Polyclonal antibody (Product # PA5-87543). Blue: DAPI for nuclear staining.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 3 SIRT7 knockdown increases the risk of Mycobacteria infection, while overexpression of SIRT7 protects cells from Mycobacteria. (A-D) Raw264.7 cells stably expressing scrambled control (NC-pLKO.1) and two independent SIRT7 shRNAs (sh1-SIRT7 and sh2-SIRT7), respectively, were established. SIRT7 expression levels in these cells were measured by quantitative RT-PCR and Western blot analysis (A) . Representative flow cytometry images (B) and percentage (C) of GFP-positive cells were recorded in the scrambled control and SIRT7-knockdown cells 24 h after infection with BCG-GFP (MOI 10:1). (D) Colony-forming unit (CFU) counts in scrambled control and SIRT7-knockdown cells after 48h infection with H37Rv. (E-H) Raw264.7 cells stably overexpressing SIRT7 (OE-SIRT7) and vector control (NC-LV6) were established. SIRT7 expression levels in these cells were measured by quantitative RT-PCR and Western blot analysis (E) . Representative flow cytometry images (F) and percentage (G) of GFP-positive cells were recorded in control and SIRT7-overexpressing cells 24 h after infection with BCG-GFP (MOI 10:1). (H) CFU counts in vector control and SIRT7-overexpressing cells after 48h infected with H37Rv. Data are representative of three independent experiments with similar results and are presented as means +- SD. One way ANOVA was performed in (A, C, D) , Unpaired Student's t-test was used in (E, G, H) . *p < 0.05; **p < 0.01; ***p < 0.001; ****p< 0.0001.