Antibody data
- Antibody Data
- Antigen structure
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- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- AM60069PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Product name
- anti LRP4 (26-350, Extracell. Dom.)
- Antibody type
- Monoclonal
- Antigen
- Fusion protein amino acids 26- 350 (Extracellular, N-terminus)Â of mouse LRP4
- Reactivity
- Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- S207-27
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
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Supportive validation
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- Acris Antibodies GmbH (provider)
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- Experimental details
- Western blot of rat brain membrane lysates showing the detection of ~215 kDa LRP4 protein using anti-LRP4 antibody [clone S207-27] Cat.-No. AM60069PU-N. Lane 1: MW ladder. Lane 2: Anti-LRP4 (1:500). Lane 3: Anti-LRP4 (1:1000). Lane 4: Anti-LRP4 (1:2000). Load: 33 µg RBM per lane. Block: 5% milk + TBST O/N at 4°C. Primary antibody: Mouse anti-LRP4 antibody [clone S207-27] at 1:500,1:1000 1:2000 dilution in TBS for 60 min at RT. Secondary antibody: Goat anti-mouse HRP antibody at 1:50 for 60 min at RT. Colour Development: TMB solution for 10 min at RT. Predicted/Observed size: ~ 215 kDa. Other band(s): none.