PA5-120636

antibody from Invitrogen Antibodies

Targeting: GTF3C3 TFIIIC102, TFiiiC2-102

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-120636

Provider

Invitrogen Antibodies

Product name

GTF3C3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive test controls include: HepG2, HeLa, 293T, RAW264.7, NIH/3T3, Mouse lung. Immunogen sequence: MSGFSPELID YLEGKISFEE FERRREERKT REKKSLQEKG KLSAEENPDD SEVPSSSGIN STKSQDKDVN EGETSDGVRK SVHKVFASML GENEDDEEEE EEEEEEEEEE ETPEQPTAGD VFVLEMVLNR ETKKMMKEKR PRSKLPRALR GLMGEANIRF

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.98 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of GTF3C3 in various cell lines using a GTF3C3 Polyclonal antibody (Product # PA5-120636) at a dilution of 1:2,000. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution. Chemiluminescent detection was performed for 10s.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of GTF3C3 in H9C2 cells using a GTF3C3 Polyclonal antibody (Product # PA5-120636). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of GTF3C3 in H9C2 cells using a GTF3C3 Polyclonal antibody (Product # PA5-120636). Blue: DAPI for nuclear staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of GTF3C3 in mouse brain using a Polyclonal antibody (Product # PA5-120636) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of GTF3C3 in mouse brain using a Polyclonal antibody (Product # PA5-120636) at a dilution of 1:100.