Explore
Validate
LearnMA5-42938
antibody from Invitrogen Antibodies
Targeting: SF3B1
Hsh155, Prp10, PRPF10, SAP155, SF3b155
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA5-42938 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SF3B1 Recombinant Rabbit Monoclonal Antibody (4A3G9)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Positive test controls include: HeLa, NIH/3T3, Jurkat, Mouse brain.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 4A3G9
- Vial size
- 100 µL
- Concentration
- 0.27 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of SF3B1 in various cell lines using a SF3B1 Recombinant Monoclonal antibody (Product # MA5-42938) at a dilution of 1:1,000. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution. Chemiluminescent detection was performed for 30s.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of SF3B1 in C6 cells using a SF3B1 Recombinant Monoclonal antibody (Product # MA5-42938). Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of SF3B1 in human appendix using a Recombinant Monoclonal antibody (Product # MA5-42938) at a dilution of 1:100. Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol..
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of SF3B1 in human appendix using a Recombinant Monoclonal antibody (Product # MA5-42938) at a dilution of 1:100. Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol..
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of SF3B1 in human appendix using a Recombinant Monoclonal antibody (Product # MA5-42938) at a dilution of 1:100. Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol..
