13720-1-AP

antibody from Invitrogen Antibodies

Targeting: BOLL BOULE

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

13720-1-AP

Provider

Invitrogen Antibodies

Product name

BOULE Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MQTDSLSPS PNPVSPVPLN NPTSAPRYGT VIPNRIFVGG IDFKTNESDL RKFFSQYGSV KEVKIVNDRA GVSKGYGFVT FETQEDAQKI LQEAEKLNYK DKKLNIGPAI RKQQVGIPRS SIMPAAGTMY LTTSTGYPYT YHNGVAYFHT PEVTSVPPPW PSRSVCSSPV MVAQPIYQQP AYHYQATTQY LPGQWQWSVP QPSASSAPFL YLQPSEVIYQ PVEIAQDGGC VPPPLSLMET SVPEPYSDHG VQATYHQVYA PSAITMPAPV MQPEPIKTVW SIHY (1-283 aa encoded by BC033674)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.13 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse testis tissue were subjected to SDS PAGE followed by western blot with 13720-1-AP (BOULE antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse ovary tissue slide using 13720-1-AP ( BOULE antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using BOULE antibody (Product # 13720-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse ovary tissue slide using 13720-1-AP (BOULE antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human testis using 13720-1-AP (BOULE antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human testis using 13720-1-AP (BOULE antibody) at dilution of 1:50 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-BOULE (IP:13720-1-AP, 4ug; Detection:13720-1-AP 1:1000) with mouse testis tissue lysate 4000ug.