HPA027821

antibody from Atlas Antibodies

Targeting: CKAP2 FLJ10749, LB1, se20-10, TMAP

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA027821

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA027821, RRID:AB_10602208

Product name

Anti-CKAP2

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

VKYWICLALIEPITSPIENIIAIYEKAILAGAQPI
EEMRHTIVDILTMKSQEKANLGENMEKSCASKEEV
KEVSIEDTGVDVDPEKLEMESKLHRNLLFQDCEKE
QDNKTKDPTH


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human testis and skeletal muscle tissues using HPA027821 antibody. Corresponding CKAP2 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human testis shows cytoplasmic and nuclear positivity in cells in seminiferous ducts.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human testis shows moderate positivity.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human bone marrow shows moderate cytoplasmic positivity in hematopoietic cells.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human skeletal muscle shows no positivity in myocytes as expected.

Sample type

HUMAN