MA5-34670

antibody from Invitrogen Antibodies

Targeting: SYNE1 8B, ARCA1, C6orf98, CPG2, dJ45H2.2, enaptin, KIAA0796, MYNE1, Nesp1, Nesprin-1, SCAR8, SYNE-1B

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-34670

Provider

Invitrogen Antibodies

Product name

Nesprin 1 Recombinant Rabbit Monoclonal Antibody (JB35-26)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Positive Control: A549, mouse spleen tissue lysate, C2C12, HUVEC, rat brain tissue, human tonsil tissue, human colon cancer tissue, human kidney tissue, mouse fallopian tubes tissue, Daudi.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

JB35-26

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles, store in dark

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Nesprin 1 in Lane 1: A549, Lane 2: Mouse spleen tissue. Samples were incubated with Nesprin 1 monoclonal antibody (Product # MA5-34670), at a dilution of 1:500.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Nesprin 1 in HUVEC cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Nesprin 1 monoclonal antibody (Product # MA5-34670), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Nesprin 1 in C2C12 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Nesprin 1 monoclonal antibody (Product # MA5-34670), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Nesprin 1 in A549 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Nesprin 1 monoclonal antibody (Product # MA5-34670), followed by DAPI (blue).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Nesprin 1 in paraffin-embedded rat brain tissue. Samples were incubated with Nesprin 1 monoclonal antibody (Product # MA5-34670), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Nesprin 1 in paraffin-embedded mouse fallopian tubes tissue. Samples were incubated with Nesprin 1 monoclonal antibody (Product # MA5-34670), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Nesprin 1 in paraffin-embedded human tonsil tissue. Samples were incubated with Nesprin 1 monoclonal antibody (Product # MA5-34670), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Nesprin 1 in paraffin-embedded human kidney tissue. Samples were incubated with Nesprin 1 monoclonal antibody (Product # MA5-34670), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Nesprin 1 in paraffin-embedded human colon cancer tissue. Samples were incubated with Nesprin 1 monoclonal antibody (Product # MA5-34670), and followed by hematoxylin.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of Nesprin 1 in Daudi cells (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Samples were incubated with Nesprin 1 monoclonal antibody (Product # MA5-34670) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.