MA5-34670
antibody from Invitrogen Antibodies
Targeting: SYNE1
8B, ARCA1, C6orf98, CPG2, dJ45H2.2, enaptin, KIAA0796, MYNE1, Nesp1, Nesprin-1, SCAR8, SYNE-1B
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [3]
- Immunohistochemistry [5]
- Flow cytometry [1]
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Validation data
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- Product number
- MA5-34670 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Nesprin 1 Recombinant Rabbit Monoclonal Antibody (JB35-26)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Positive Control: A549, mouse spleen tissue lysate, C2C12, HUVEC, rat brain tissue, human tonsil tissue, human colon cancer tissue, human kidney tissue, mouse fallopian tubes tissue, Daudi.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- JB35-26
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles, store in dark
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Nesprin 1 in Lane 1: A549, Lane 2: Mouse spleen tissue. Samples were incubated with Nesprin 1 monoclonal antibody (Product # MA5-34670), at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Nesprin 1 in HUVEC cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Nesprin 1 monoclonal antibody (Product # MA5-34670), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Nesprin 1 in C2C12 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Nesprin 1 monoclonal antibody (Product # MA5-34670), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Nesprin 1 in A549 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Nesprin 1 monoclonal antibody (Product # MA5-34670), followed by DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Nesprin 1 in paraffin-embedded rat brain tissue. Samples were incubated with Nesprin 1 monoclonal antibody (Product # MA5-34670), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Nesprin 1 in paraffin-embedded mouse fallopian tubes tissue. Samples were incubated with Nesprin 1 monoclonal antibody (Product # MA5-34670), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Nesprin 1 in paraffin-embedded human tonsil tissue. Samples were incubated with Nesprin 1 monoclonal antibody (Product # MA5-34670), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Nesprin 1 in paraffin-embedded human kidney tissue. Samples were incubated with Nesprin 1 monoclonal antibody (Product # MA5-34670), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Nesprin 1 in paraffin-embedded human colon cancer tissue. Samples were incubated with Nesprin 1 monoclonal antibody (Product # MA5-34670), and followed by hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of Nesprin 1 in Daudi cells (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Samples were incubated with Nesprin 1 monoclonal antibody (Product # MA5-34670) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.