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LearnH00008837-M01
antibody from Novus Biologicals
Targeting: CFLAR
c-FLIP, CASH, CASP8AP1, Casper, cFLIP, CLARP, FLAME, FLIP, I-FLICE, MRIT
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [4]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- H00008837-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00008837-M01, RRID:AB_537716
- Product name
- Mouse Monoclonal FLIP Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. CFLAR - CASP8 and FADD-like apoptosis regulator
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references miR-150-504-519d inhibits the growth of human colorectal cancer cell line SW48 and downregulates c-FLIP receptor.
Rong G, Yang X, Wu H, Wu Y
Journal of cellular biochemistry 2018 Dec 12;
Journal of cellular biochemistry 2018 Dec 12;
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: FLIP Antibody (2F12) [H00008837-M01] - Western blot of FLIP provided courtesty of verified customer.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: FLIP Antibody (2F12) [H00008837-M01] - Analysis of CFLAR expression in transfected 293T cell line by CFLAR monoclonal antibody (M01), clone 2F12.Lane 1: CFLAR transfected lysate (Predicted MW: 55.3 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: FLIP Antibody (2F12) [H00008837-M01] - CFLAR monoclonal antibody (M01), clone 2F12 Analysis of CFLAR expression in IMR-32.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: FLIP Antibody (2F12) [H00008837-M01] - CFLAR monoclonal antibody (M01), clone 2F12. Analysis of CFLAR expression in K-562.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: FLIP Antibody (2F12) [H00008837-M01] - Detection limit for recombinant GST tagged CFLAR is approximately 0.3ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: FLIP Antibody (2F12) [H00008837-M01] - Analysis of monoclonal antibody to CFLAR on HeLa cell. Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: FLIP Antibody (2F12) [H00008837-M01] - Analysis of monoclonal antibody to CFLAR on formalin-fixed paraffin-embedded human kidney. Antibody concentration 3 ug/ml.
