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Learn10394-1-AP
antibody from Invitrogen Antibodies
Targeting: CFLAR
c-FLIP, CASH, CASP8AP1, Casper, cFLIP, CLARP, FLAME, FLIP, I-FLICE, MRIT
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [2]
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Validation data
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- Product number
- 10394-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CFLAR/FLIP Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: DVVPPNVRD LLDILRERGK LSVGDLAELL YRVRRFDLLK RILKMDRKAV ETHLLRNPHL VSDYRVLMAE IGEDLDKSDV SSLIFLMKDY MGRGKISKEK SFLDLVVELE KLNLVAPDQL DLLEKCLKNI HRIDLKTKIQ KYKQSVQGAG TSYRN (31-184 aa encoded by B C001602)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.72 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 10394-1-AP (CFLAR/FLIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- WB result of CFLAR/FLIP antibody (10394-1-AP; 1:500; incubated at room temperature for 1.5 hours) with sh-Control and sh-CFLAR/FLIP transfected HepG2 cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 10394-1-AP (CFLAR/FLIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HeLa cells using 10394-1-AP (CFLAR/FLIP antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human skeletal muscle slide using 10394-1-AP ( CFLAR/FLIP antibody at dilution of 1:50.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human skeletal muscle slide using 10394-1-AP ( CFLAR/FLIP antibody at dilution of 1:50.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-CFLAR/FLIP (IP:10394-1-AP, 4 ug; Detection:10394-1-AP 1:500) with Jurkat cells lysate 4000 ug.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-CFLAR/FLIP (IP:10394-1-AP, 4ug; Detection:10394-1-AP 1:500) with Jurkat cells lysate 4000ug.
