PAB21038

antibody from Abnova Corporation

Targeting: NOP58 HSPC120, NOP5

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB21038

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB21038, RRID:AB_10965474

Product name

NOP5/NOP58 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant NOP5/NOP58.

Antigen sequence

MLVLFETSVGYAIFKVLNEKKLQEVDSLWKEFETP
EKANKIVKLKHFEKFQDTAEALAAFTALMEGKINK
QLKKVLKKIVKEAHEPLAVADAKLGGVIKEKLNLS
CIHSPVVNELMRGIRSQMDGL


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with NOP5/NOP58 polyclonal antibody (Cat # PAB21038) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 with NOP5/NOP58 polyclonal antibody (Cat # PAB21038) at 1-4 ug/mL dilution shows positivity in nucleus and nucleoli.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human esophagus with NOP5/NOP58 polyclonal antibody (Cat # PAB21038) shows strong nuclear positivity, seen as distinctly stained nucleoli, in squamous epithelial cells at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)