Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 302903 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#302903, RRID:AB_314305
- Product name
- Biotin anti-human CD28
- Antibody type
- Monoclonal
- Antigen
- CD28
- Description
- CD28 is a 44 kD disulfide-linked homodimeric type I glycoprotein. It is a member of the immunoglobulin superfamily and is also known as T44 or Tp44. CD28 is expressed on most T lineage cells, NK cell subsets, and plasma cells. CD28 binds both CD80 and CD86 using a highly conserved motif MYPPY in the CDR3-like loop. CD28 is considered a major co-stimulatory molecule, inducing T lymphocyte activation and IL-2 synthesis, and preventing cell death. In vitro studies indicate that ligation of CD28 on T cells by CD80 and CD86 on antigen presenting cells provides a costimulatory signal required for T cell activation and proliferation.
- Reactivity
- Human, Simian
- Host
- Mouse
- Conjugate
- Biotin
- Isotype
- IgG
- Vial size
- 25 µg
- Concentration
- 0.5 mg/ml
- Storage
- The antibody solution should be stored undiluted at 4°C. Do not freeze.
Submitted references HIV-1 Nef disrupts intracellular trafficking of major histocompatibility complex class I, CD4, CD8, and CD28 by distinct pathways that share common elements.
Burkholderia pseudomallei infection of T cells leads to T-cell costimulation partially provided by flagellin.
Human amnion mesenchyme harbors cells with allogeneic T-cell suppression and stimulation capabilities.
Ex vivo simulation of drug action: quantification of drug-induced mRNA as a bridge between preclinical and clinical trials.
A major role of PKC theta and NFkappaB in the regulation of hTERT in human T lymphocytes.
Leonard JA, Filzen T, Carter CC, Schaefer M, Collins KL
Journal of virology 2011 Jul;85(14):6867-81
Journal of virology 2011 Jul;85(14):6867-81
Burkholderia pseudomallei infection of T cells leads to T-cell costimulation partially provided by flagellin.
Ye Z, Lee CM, Sun GW, Gan YH
Infection and immunity 2008 Jun;76(6):2541-50
Infection and immunity 2008 Jun;76(6):2541-50
Human amnion mesenchyme harbors cells with allogeneic T-cell suppression and stimulation capabilities.
Magatti M, De Munari S, Vertua E, Gibelli L, Wengler GS, Parolini O
Stem cells (Dayton, Ohio) 2008 Jan;26(1):182-92
Stem cells (Dayton, Ohio) 2008 Jan;26(1):182-92
Ex vivo simulation of drug action: quantification of drug-induced mRNA as a bridge between preclinical and clinical trials.
Mitsuhashi M
Clinical chemistry 2007 Jan;53(1):148-9
Clinical chemistry 2007 Jan;53(1):148-9
A major role of PKC theta and NFkappaB in the regulation of hTERT in human T lymphocytes.
Sheng WY, Chen YR, Wang TC
FEBS letters 2006 Dec 22;580(30):6819-24
FEBS letters 2006 Dec 22;580(30):6819-24
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Supportive validation
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- BioLegend (provider)
- Main image
- Experimental details
- Human peripheral blood lymphocytes stained with biotinylated CD28.2, followed by Sav-PE
- Conjugate
- Biotin