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Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- MAB434-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse CTLA-4 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse CTLA-4 in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with recombinant mouse (rm) CD28, recombinant human CTLA-4, rmICOS, or rmPD-1.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
XP_001479180- Isotype
- IgG
- Antibody clone number
- 63828
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Dopamine, through the extracellular signal-regulated kinase pathway, downregulates CD4+CD25+ regulatory T-cell activity: implications for neurodegeneration.
Kipnis J, Cardon M, Avidan H, Lewitus GM, Mordechay S, Rolls A, Shani Y, Schwartz M
The Journal of neuroscience : the official journal of the Society for Neuroscience 2004 Jul 7;24(27):6133-43
The Journal of neuroscience : the official journal of the Society for Neuroscience 2004 Jul 7;24(27):6133-43
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- CTLA-4 Inhibition of B7-1/CD80-induced IL-2 Secretion and Neutralization by Mouse CTLA-4 Antibody. Recombinant Mouse CTLA-4 Fc Chimera (Catalog # 434-CT) inhibits Recombinant Human B7-1/CD80 Fc Chimera (Catalog # 140-B1) induced IL-2 secretion in the Jurkat human acute T cell leukemia cell line in a dose-dependent manner (orange line), as measured by the Human IL-2 Quantikine ELISA Kit (Catalog # D2050). Inhibition of Recombinant Human B7-1/CD80 Fc Chimera (3 µg/mL) activity elicited by Recombinant Mouse CTLA-4 Fc Chimera (1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse CTLA-4 Monoclonal Antibody (Catalog # MAB434). The ND50 is typically 2.5-10 µg/mL in the presence of PHA (10 µg/mL).
