PAB20387

antibody from Abnova Corporation

Targeting: KDM4A JHDM3A, JMJD2, JMJD2A, KIAA0677, TDRD14A

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB20387

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB20387, RRID:AB_10965829

Product name

JMJD2A polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant JMJD2A.

Antigen sequence

DVFVRKFQPERYKLWKAGKDNTVIDHTLPTPEAAE
FLKESELPPRAGNEEECPEEDMEGVEDGEEGDLKT
SLAKHRIGTKRHRVCLEIPQEVSQSELFPKEDLSS
EQYEMTEC


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with JMJD2A polyclonal antibody (Cat # PAB20387) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with JMJD2A polyclonal antibody (Cat # PAB20387) at 1-4 ug/mL dilution shows positivity in nucleoli.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human testis with JMJD2A polyclonal antibody (Cat # PAB20387) shows strong cytoplasmic positivity in cells in seminiferus ducts.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)