NB110-40585

antibody from Novus Biologicals

Targeting: KDM4A JHDM3A, JMJD2, JMJD2A, KIAA0677, TDRD14A

Provider product page for NB110-40585

Western blot

Immunocytochemistry

Immunoprecipitation

Antibody data

Antibody Data
Antigen structure
References [12]
Comments [0]
Validations
Western blot [3]
Immunoprecipitation [1]

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Product number: NB110-40585 - Provider product page
Provider: Novus Biologicals
Proper citation: Novus Cat#NB110-40585, RRID:AB_669535
Product name: Rabbit Polyclonal Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody
Antibody type: Polyclonal
Description: Immunogen affinity purified.
Reactivity: Human, Mouse
Host: Rabbit
Isotype: IgG
Vial size: 0.1 ml
Concentration: 0.2 mg/ml
Storage: Store at 4C. Do not freeze.

KDM4A protein structure - NB110-40585 shown in red.

Submitted references SHMT2 and the BRCC36/BRISC deubiquitinase regulate HIV-1 Tat K63-ubiquitylation and destruction by autophagy.

Xu M, Moresco JJ, Chang M, Mukim A, Smith D, Diedrich JK, Yates JR 3rd, Jones KA
PLoS pathogens 2018 May;14(5):e1007071

The oncometabolite 2-hydroxyglutarate activates the mTOR signalling pathway.

Carbonneau M, M Gagné L, Lalonde ME, Germain MA, Motorina A, Guiot MC, Secco B, Vincent EE, Tumber A, Hulea L, Bergeman J, Oppermann U, Jones RG, Laplante M, Topisirovic I, Petrecca K, Huot MÉ, Mallette FA
Nature communications 2016 Sep 14;7:12700

KDM4B and KDM4A promote endometrial cancer progression by regulating androgen receptor, c-myc, and p27kip1.

Qiu MT, Fan Q, Zhu Z, Kwan SY, Chen L, Chen JH, Ying ZL, Zhou Y, Gu W, Wang LH, Cheng WW, Zeng J, Wan XP, Mok SC, Wong KK, Bao W
Oncotarget 2015 Oct 13;6(31):31702-20

Lamin A/C-dependent interaction with 53BP1 promotes cellular responses to DNA damage.

Gibbs-Seymour I, Markiewicz E, Bekker-Jensen S, Mailand N, Hutchison CJ
Aging cell 2015 Apr;14(2):162-9

JMJD2A attenuation affects cell cycle and tumourigenic inflammatory gene regulation in lipopolysaccharide stimulated neuroectodermal stem cells.

Das A, Chai JC, Jung KH, Das ND, Kang SC, Lee YS, Seo H, Chai YG
Experimental cell research 2014 Nov 1;328(2):361-78

JMJD2A promotes cellular transformation by blocking cellular senescence through transcriptional repression of the tumor suppressor CHD5.

Mallette FA, Richard S
Cell reports 2012 Nov 29;2(5):1233-43

Histone demethylase JMJD2C is a coactivator for hypoxia-inducible factor 1 that is required for breast cancer progression.

Luo W, Chang R, Zhong J, Pandey A, Semenza GL
Proceedings of the National Academy of Sciences of the United States of America 2012 Dec 4;109(49):E3367-76

The JMJD2A demethylase regulates apoptosis and proliferation in colon cancer cells.

Kim TD, Shin S, Berry WL, Oh S, Janknecht R
Journal of cellular biochemistry 2012 Apr;113(4):1368-76

RNF8- and RNF168-dependent degradation of KDM4A/JMJD2A triggers 53BP1 recruitment to DNA damage sites.

Mallette FA, Mattiroli F, Cui G, Young LC, Hendzel MJ, Mer G, Sixma TK, Richard S
The EMBO journal 2012 Apr 18;31(8):1865-78

SCF(FBXO22) regulates histone H3 lysine 9 and 36 methylation levels by targeting histone demethylase KDM4A for ubiquitin-mediated proteasomal degradation.

Tan MK, Lim HJ, Harper JW
Molecular and cellular biology 2011 Sep;31(18):3687-99

Alterations of histone modifications by cobalt compounds.

Li Q, Ke Q, Costa M
Carcinogenesis 2009 Jul;30(7):1243-51

Activation of androgen receptor by histone demethylases JMJD2A and JMJD2D.

Shin S, Janknecht R
Biochemical and biophysical research communications 2007 Aug 3;359(3):742-6

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Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NB110-40585] - Detection of Human and Mouse Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A on HeLa whole cell lysate using NB110-40585. Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A was also immunoprecipitated by rabbit anti-Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A antibody NB110-40584.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NB110-40585] - Nuclear extract (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-JMJD2A antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NB110-40585] - Lamins A/C-53BP1 interaction is dependent on the 53BP1 Tudor domain. U2OS/GFP-lamin A cells were subjected to IR (10 Gy) and allowed to recover for 1 h. Cell extracts were then subjected to immunoprecipitation using GFP-Trap beads, and bound complexes were then analyzed by immunoblotting using GFP, LAP2alpha and JMJD2A antibodies. WCE represents 1% input. Image collected and cropped by CiteAb from the following publication (http://doi.wiley.com/10.1111/acel.12258), licensed under a CC-BY licence.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunoprecipitation: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NB110-40585] - Detection of human Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A by western blot of immunoprecipitates. Samples: Nuclear Extract (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A antibody NB110-40585 used for IP at 6 ug per reaction. Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A was also immunoprecipitated by rabbit anti-Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A antibody NB110-40584. For blotting immunoprecipitated Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A, NB110-40585 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.