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Western blot
Proximity ligation assayAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- ELISA [1]
- Immunoprecipitation [1]
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- Product number
- H00007855-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00007855-M01, RRID:AB_2109107
- Product name
- Mouse Monoclonal Frizzled-5 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. FZD5 - frizzled homolog 5 (Drosophila)
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Frizzled-5: a high affinity receptor for secreted frizzled-related protein-2 activation of nuclear factor of activated T-cells c3 signaling to promote angiogenesis.
Peterson YK, Nasarre P, Bonilla IV, Hilliard E, Samples J, Morinelli TA, Hill EG, Klauber-DeMore N
Angiogenesis 2017 Nov;20(4):615-628
Angiogenesis 2017 Nov;20(4):615-628
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Frizzled-5 Antibody (6A3) [H00007855-M01] - Analysis of FZD5 expression in transfected 293T cell line by FZD5 monoclonal antibody (M01), clone 6A3.Lane 1: FZD5 transfected lysate(64.5 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Frizzled-5 Antibody (6A3) [H00007855-M01] - Detection limit for recombinant GST tagged FZD5 is approximately 0.3ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Frizzled-5 Antibody (6A3) [H00007855-M01] - Analysis of FZD5 transfected lysate using anti-FZD5 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with FZD5 MaxPab rabbit polyclonal antibody.
