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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [10]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- NB100-57852 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-57852, RRID:AB_2283160
- Product name
- Goat Polyclonal P4HA1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This antibody is expected to recognise both reported isoforms (NP_000908.2 and NP_001017962.1).
- Reactivity
- Human, Mouse
- Host
- Goat
- Antigen sequence
DFARKDEPDAFKE- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Prolyl 4-hydroxylase subunit alpha 1 (P4HA1) is a biomarker of poor prognosis in primary melanomas, and its depletion inhibits melanoma cell invasion and disrupts tumor blood vessel walls.
In vitro chondrogenic potency of surplus chondrocytes from autologous transplantation procedures does not predict short-term clinical outcomes.
Discoidin domain receptor 2 germline gene deletion leads to altered heart structure and function in the mouse.
Ganetespib blocks HIF-1 activity and inhibits tumor growth, vascularization, stem cell maintenance, invasion, and metastasis in orthotopic mouse models of triple-negative breast cancer.
Hypoxia-inducible factor 1 mediates TAZ expression and nuclear localization to induce the breast cancer stem cell phenotype.
Collagen prolyl hydroxylases are essential for breast cancer metastasis.
Hypoxia-inducible factor 1 (HIF-1) promotes extracellular matrix remodeling under hypoxic conditions by inducing P4HA1, P4HA2, and PLOD2 expression in fibroblasts.
Intracellular calreticulin regulates multiple steps in fibrillar collagen expression, trafficking, and processing into the extracellular matrix.
Cloning of the human prolyl 4-hydroxylase alpha subunit isoform alpha(II) and characterization of the type II enzyme tetramer. The alpha(I) and alpha(II) subunits do not form a mixed alpha(I)alpha(II)beta2 tetramer.
Cloning of the human prolyl 4-hydroxylase alpha subunit isoform alpha(II) and characterization of the type II enzyme tetramer. The alpha(I) and alpha(II) subunits do not form a mixed alpha(I)alpha(II)beta2 tetramer.
Eriksson J, Le Joncour V, Jahkola T, Juteau S, Laakkonen P, Saksela O, Hölttä E
Molecular oncology 2020 Apr;14(4):742-762
Molecular oncology 2020 Apr;14(4):742-762
In vitro chondrogenic potency of surplus chondrocytes from autologous transplantation procedures does not predict short-term clinical outcomes.
Islam A, Fossum V, Hansen AK, Urbarova I, Knutsen G, Martinez-Zubiaurre I
BMC musculoskeletal disorders 2019 Jan 10;20(1):19
BMC musculoskeletal disorders 2019 Jan 10;20(1):19
Discoidin domain receptor 2 germline gene deletion leads to altered heart structure and function in the mouse.
Cowling RT, Yeo SJ, Kim IJ, Park JI, Gu Y, Dalton ND, Peterson KL, Greenberg BH
American journal of physiology. Heart and circulatory physiology 2014 Sep 1;307(5):H773-81
American journal of physiology. Heart and circulatory physiology 2014 Sep 1;307(5):H773-81
Ganetespib blocks HIF-1 activity and inhibits tumor growth, vascularization, stem cell maintenance, invasion, and metastasis in orthotopic mouse models of triple-negative breast cancer.
Xiang L, Gilkes DM, Chaturvedi P, Luo W, Hu H, Takano N, Liang H, Semenza GL
Journal of molecular medicine (Berlin, Germany) 2014 Feb;92(2):151-64
Journal of molecular medicine (Berlin, Germany) 2014 Feb;92(2):151-64
Hypoxia-inducible factor 1 mediates TAZ expression and nuclear localization to induce the breast cancer stem cell phenotype.
Xiang L, Gilkes DM, Hu H, Takano N, Luo W, Lu H, Bullen JW, Samanta D, Liang H, Semenza GL
Oncotarget 2014 Dec 30;5(24):12509-27
Oncotarget 2014 Dec 30;5(24):12509-27
Collagen prolyl hydroxylases are essential for breast cancer metastasis.
Gilkes DM, Chaturvedi P, Bajpai S, Wong CC, Wei H, Pitcairn S, Hubbi ME, Wirtz D, Semenza GL
Cancer research 2013 Jun 1;73(11):3285-96
Cancer research 2013 Jun 1;73(11):3285-96
Hypoxia-inducible factor 1 (HIF-1) promotes extracellular matrix remodeling under hypoxic conditions by inducing P4HA1, P4HA2, and PLOD2 expression in fibroblasts.
Gilkes DM, Bajpai S, Chaturvedi P, Wirtz D, Semenza GL
The Journal of biological chemistry 2013 Apr 12;288(15):10819-29
The Journal of biological chemistry 2013 Apr 12;288(15):10819-29
Intracellular calreticulin regulates multiple steps in fibrillar collagen expression, trafficking, and processing into the extracellular matrix.
Van Duyn Graham L, Sweetwyne MT, Pallero MA, Murphy-Ullrich JE
The Journal of biological chemistry 2010 Mar 5;285(10):7067-78
The Journal of biological chemistry 2010 Mar 5;285(10):7067-78
Cloning of the human prolyl 4-hydroxylase alpha subunit isoform alpha(II) and characterization of the type II enzyme tetramer. The alpha(I) and alpha(II) subunits do not form a mixed alpha(I)alpha(II)beta2 tetramer.
Annunen P, Helaakoski T, Myllyharju J, Veijola J, Pihlajaniemi T, Kivirikko KI
The Journal of biological chemistry 1997 Jul 11;272(28):17342-8
The Journal of biological chemistry 1997 Jul 11;272(28):17342-8
Cloning of the human prolyl 4-hydroxylase alpha subunit isoform alpha(II) and characterization of the type II enzyme tetramer. The alpha(I) and alpha(II) subunits do not form a mixed alpha(I)alpha(II)beta2 tetramer.
Annunen P, Helaakoski T, Myllyharju J, Veijola J, Pihlajaniemi T, Kivirikko KI
The Journal of biological chemistry 1997 Jul 11;272(28):17342-8
The Journal of biological chemistry 1997 Jul 11;272(28):17342-8
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: P4HA1 Antibody [NB100-57852] - Analysis of P4HA1 in HEK293 cell lysate (35ug protein in RIPA buffer) using NB100-57852 at 0.1ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: P4HA1 Antibody [NB100-57852] - Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: P4HA1 Antibody [NB100-57852] - Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
