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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [3]
- Other assay [1]
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- Product number
- PA5-55353 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-P4HA1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: DYYHTELWME QALRQLDEGE ISTIDKVSVL DYLSYAVYQQ GDLDKALLLT KKLLELDPEH QRANGNLKYF EYIMAKEKDV NKSASDDQSD QKTTPKKKGV AVDYLPERQK YEMLCRGEGI KMTPRRQKKL FCRYHDGNRN PK Highest antigen sequence identity to the following orthologs: Mouse - 92%, Rat - 93%.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.3 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of P4HA1 in human cell lines PC-3 and HEK293 using a P4HA1 Polyclonal Antibody (Product # PA5-55353). Corresponding P4HA1 RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of P4HA1 using P4HA1 Polyclonal Antibody (Product # PA5-55353 ) (A) shows similar pattern to an independent P4HA1 Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of P4HA1 in mouse cell line NIH-3T3 and rat cell line NBT-II using a P4HA1 Polyclonal Antibody (Product # PA5-55353).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of P4HA1 in human testis using a P4HA1 Polyclonal Antibody (Product # PA5-55353) shows strong cytoplasmic and nuclear positivity in cells in seminiferous ducts and Leydig cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of P4HA1 in human placenta using P4HA1 Polyclonal Antibody (Product # PA5-55353) shows high expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of P4HA1 in human pancreas using P4HA1 Polyclonal Antibody (Product # PA5-55353) shows low expression as expected.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of P4HA1 in human cell line U-2 OS shows positivity in endoplasmic reticulum & vesicles. Samples were probed using a P4HA1 Polyclonal Antibody (Product # PA5-55353).
