NBP1-84398

antibody from Novus Biologicals

Targeting: P4HA1 C-P4Halpha(I), P4HA

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-84398

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-84398, RRID:AB_11015709

Product name

Rabbit Polyclonal P4HA1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specificity of human P4HA1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Prolyl 4-hydroxylase subunit alpha 1 (P4HA1) is a biomarker of poor prognosis in primary melanomas, and its depletion inhibits melanoma cell invasion and disrupts tumor blood vessel walls.

Eriksson J, Le Joncour V, Jahkola T, Juteau S, Laakkonen P, Saksela O, Hölttä E
Molecular oncology 2020 Apr;14(4):742-762

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: P4HA1 Antibody [NBP1-84398] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: P4HA1 Antibody [NBP1-84398] - Analysis in human cell lines PC-3 and HEK293 using Anti-P4HA1 antibody. Corresponding P4HA1 RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: P4HA1 Antibody [NBP1-84398] - Analysis using Anti-P4HA1 antibody NBP1-84398 (A) shows similar pattern to independent antibody NBP1-84397 (B).

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: P4HA1 Antibody [NBP1-84398] - Staining of human pancreas shows low expression as expected.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: P4HA1 Antibody [NBP1-84398] - Staining of human placenta shows high expression.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: P4HA1 Antibody [NBP1-84398] - Staining of human cerebellum shows moderate granular cytoplasmic positivity in Purkinje cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: P4HA1 Antibody [NBP1-84398] - Staining of human endometrium shows strong cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: P4HA1 Antibody [NBP1-84398] - Staining of human pancreas shows very weak positivity in exocrine glandular cells as expected.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: P4HA1 Antibody [NBP1-84398] - Staining of human placenta shows strong cytoplasmic positivity in decidual cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: P4HA1 Antibody [NBP1-84398] - Staining of human testis shows moderate to strong granular cytoplasmic positivity in cells in seminiferous ducts.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: P4HA1 Antibody [NBP1-84398] - Staining in human placenta and pancreas tissues using NBP1-84398 antibody. Corresponding P4HA1 RNA-seq data are presented for the same tissues.