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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- PA5-29635 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MYL1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa, mouse heart, rat muscle.
- Concentration
- 0.28 mg/mL
Submitted references Valsartan inhibits RhoA-ROCK2-MYL pathway in rat model of alcoholic cardiomyopathy.
Li L, Jing L, Zhao J, Lv J, Yang W, Li W, Zhou L
Experimental and therapeutic medicine 2019 Dec;18(6):4313-4321
Experimental and therapeutic medicine 2019 Dec;18(6):4313-4321
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Fast skeletal myosin light chain 1 using 30 µg of HeLa lysate. Samples were loaded onto a 15% SDS-PAGE gel and probed with a Fast skeletal myosin light chain 1 polyclonal antibody (Product # PA5-29635) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Fast skeletal myosin light chain 1 using 1 µg of rat muscle lysate. Samples were loaded onto a 15% SDS-PAGE gel and probed with a Fast skeletal myosin light chain 1 polyclonal antibody (Product # PA5-29635) at a dilution of 1:10,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using MYL1 Polyclonal Antibody (Product # PA5-29635). Sample (whole cell lysate). Lane A: 1 µg mouse heart. Lane B: 5 µg mouse heart. 15% SDS PAGE. MYL1 Polyclonal Antibody (Product # PA5-29635) diluted at 1:10,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using MYL1 Polyclonal Antibody (Product # PA5-29635). RD whole cell extract (30 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with MYL1 Polyclonal Antibody (Product # PA5-29635) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MYL1 Polyclonal Antibody detects Fast skeletal myosin light chain 1 protein by Western blot analysis. Rat tissue extracts (1 µg) was separated by 15 % SDS-PAGE, and the membrane was blotted with MYL1 Polyclonal Antibody (Product # PA5-29635) at a dilution of 1:20,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Fast skeletal myosin light chain 1 in paraformaldehyde-fixed HeLa cells using a Fast skeletal myosin light chain 1 polyclonal antibody (Product # PA5-29635) at a 1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5. Effect of valsartan on protein expression level of RhoA, ROCK-2 and MYL in myocardial cells-amelioration by valsartan. (A) Western blots results of RhoA, ROCK-2 and MYL expression in myocardial cells. The experiment was repeated 3 times with similar results. The figure presents a representative analysis. Quantification of the western blots (B) RhoA, (C) ROCK-2 (D) and MYL. Columns are means +/- standard error of the mean, (n=3). *P
