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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- H00006950-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00006950-M01, RRID:AB_425710
- Product name
- TCP1 monoclonal antibody (M01), clone 2B2-D6
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant TCP1.
- Antigen sequence
MEGPLSVFGDRSTGETIRSQNVMAAASIANIVKSS
FGPVGLDKMLVDDIGDVTITNDGATILKLLEVEHP
AAKVLCELADLQDKEVGDGTTSVVIIAAELLKNAD
ELVKQKIHPTSVISGYRLACKEAVRYINENLIVNT
DELGRDCLINAAKTSMSSKIIGINGDFFANMVVDA
VLAIKYTDIRGQPRYPVNSVNILKAHGRSQMESML
ISGYALNCVVGSQGMPKRIVNAKIACLDFSLQKTK
MKLGVQVVITDPEKLDQIRQRESDITKERIQKILA
TGANVILTTGGIDDMCLKYFVEAGAMAVRRVLKRD
LKRIAKASGATILSTLANLEGEETFEAAMLGQAEE
VVQERICDDELILIKNTKARTSASIILRGANDFMC
DEMERSLHDALCVVKRVLESKSVVPGGGAVEAALS
IYLENYATSMGSREQLAIAEFARSLLVIPNTLAVN
AAQDSTDLVAKLRAFHNEAQVNPERKNLKWIGLDL
SNGKPRDNKQAGVFEPTIVKVKSLKFATEAAITIL
RIDDLIKLHPESKDDKHGSYEDAVHSGALND- Isotype
- IgG
- Antibody clone number
- 2B2-D6
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references BBS6, BBS10, and BBS12 form a complex with CCT/TRiC family chaperonins and mediate BBSome assembly.
Seo S, Baye LM, Schulz NP, Beck JS, Zhang Q, Slusarski DC, Sheffield VC
Proceedings of the National Academy of Sciences of the United States of America 2010 Jan 26;107(4):1488-93
Proceedings of the National Academy of Sciences of the United States of America 2010 Jan 26;107(4):1488-93
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- TCP1 monoclonal antibody (M01), clone 2B2-D6 Western Blot analysis of TCP1 expression in Hela ( Cat # L013V1 ).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged TCP1 is approximately 0.1ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to TCP1 on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to TCP1 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
