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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Western blot [2]
- Immunohistochemistry [4]
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- Product number
- NBP1-82188 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-82188, RRID:AB_11037354
- Product name
- Rabbit Polyclonal ALKBH5 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human ALKBH5 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references RNA demethylase ALKBH5 promotes ovarian carcinogenesis in a simulated tumour microenvironment through stimulating NF-κB pathway.
The m6A demethylase ALKBH5 controls trophoblast invasion at the maternal-fetal interface by regulating the stability of CYR61 mRNA.
Hypoxia-inducible factors regulate pluripotency factor expression by ZNF217- and ALKBH5-mediated modulation of RNA methylation in breast cancer cells.
Hypoxia induces the breast cancer stem cell phenotype by HIF-dependent and ALKBH5-mediated m⁶A-demethylation of NANOG mRNA.
Jiang Y, Wan Y, Gong M, Zhou S, Qiu J, Cheng W
Journal of cellular and molecular medicine 2020 Jun;24(11):6137-6148
Journal of cellular and molecular medicine 2020 Jun;24(11):6137-6148
The m6A demethylase ALKBH5 controls trophoblast invasion at the maternal-fetal interface by regulating the stability of CYR61 mRNA.
Li XC, Jin F, Wang BY, Yin XJ, Hong W, Tian FJ
Theranostics 2019;9(13):3853-3865
Theranostics 2019;9(13):3853-3865
Hypoxia-inducible factors regulate pluripotency factor expression by ZNF217- and ALKBH5-mediated modulation of RNA methylation in breast cancer cells.
Zhang C, Zhi WI, Lu H, Samanta D, Chen I, Gabrielson E, Semenza GL
Oncotarget 2016 Oct 4;7(40):64527-64542
Oncotarget 2016 Oct 4;7(40):64527-64542
Hypoxia induces the breast cancer stem cell phenotype by HIF-dependent and ALKBH5-mediated m⁶A-demethylation of NANOG mRNA.
Zhang C, Samanta D, Lu H, Bullen JW, Zhang H, Chen I, He X, Semenza GL
Proceedings of the National Academy of Sciences of the United States of America 2016 Apr 5;113(14):E2047-56
Proceedings of the National Academy of Sciences of the United States of America 2016 Apr 5;113(14):E2047-56
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ALKBH5 Antibody [NBP1-82188] - Analysis of ALKBH5 in MDA-MB-231 human breast cancer subclones using anti-ALKBH5 antibody. Image from verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ALKBH5 Antibody [NBP1-82188] - Analysis in human cell line U-87 MG.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ALKBH5 Antibody [NBP1-82188] - Staining of human liver shows weak to moderate nuclear positivity in hepatocytes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ALKBH5 Antibody [NBP1-82188] - Staining of human fallopian tube shows moderate to strong nuclear positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ALKBH5 Antibody [NBP1-82188] - Staining of human kidney shows nuclear positivity.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ALKBH5 Antibody [NBP1-82188] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.
