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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Other assay [2]
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- Product number
- PA5-37290 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SLC22A2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody detects endogenous protein at a molecular weight of 100 kDa.
- Concentration
- 1 mg/mL
Submitted references Divergent Regulation of OCT and MATE Drug Transporters by Cadmium Exposure.
Incorporation of a Biguanide Scaffold Enhances Drug Uptake by Organic Cation Transporters 1 and 2.
Yang H, Zhou S, Guo D, Obianom ON, Li Q, Shu Y
Pharmaceutics 2021 Apr 13;13(4)
Pharmaceutics 2021 Apr 13;13(4)
Incorporation of a Biguanide Scaffold Enhances Drug Uptake by Organic Cation Transporters 1 and 2.
Obianom ON, Coutinho AL, Yang W, Yang H, Xue F, Shu Y
Molecular pharmaceutics 2017 Aug 7;14(8):2726-2739
Molecular pharmaceutics 2017 Aug 7;14(8):2726-2739
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SLC22A2 in Lane 1: HeLa cell lysate, Lane 2: sp2/0 cell lysate, Lane 3: H9C2 cell lysate. Samples were incubated with SLC22A2 polyclonal antibody (Product # PA5-37290) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SLC22A2 using SLC22A2 polyclonal antibody (Product # PA5-37290) at a dilution of 1:500. Lane 1: Hela cell lysate, Lane 2: sp2/0 cell lysate, Lane 3: H9C2 cell lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 Cd 2+ exposure increases membrane expression of hOCT1 and hOCT2 but not hMATE1. ( a ) Immunoblotting (IB) analysis of total and membrane fraction of hOCT2 in HEK-hOCT2 cells with and without exposure to different concentrations of Cd 2+ for 20 min. ( b ) IB analysis of total and membrane fraction of hOCT1 in HEK-hOCT1 cells with and without exposure to Cd 2+ for 20 min. ( c ) IB analysis of total and membrane fraction of hMATE1 in HEK-hMATE1 cells with and without exposure to different concentrations of Cd 2+ for 20 min. All figures are representatives of three independent experiments. Pan-cadherin and Na + /K + ATPase are served as the positive control and beta-actin as negative control for membrane proteins.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 Effect of brefeldin (BFA) treatment on the membrane expression and function of hOCT2 and hMATE1. ( a ) Cellular uptake of metformin in HEK-hOCT2 and HEK-hMATE1 cells after exposure to BFA at indicated times. ( b ) IB analysis of total and membrane fraction of hOCT2 in HEK-hOCT2 cells after exposure to BFA at indicated times. ( c ) IB analysis of total and membrane fraction of hMATE1 in HEK-hMATE1 cells after exposure to BFA at indicated times. For b and c, pan-cadherin is served as the positive control and beta-actin as the negative control for membrane proteins. For a, data are presented as mean +- S.D. ; * p < 0.05; n.s., no significance.
