Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [3]
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Validation data
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- Product number
- PA5-85707 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BARD1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Keep as concentrated solution.
- Concentration
- 0.58 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of BARD1 in A431 whole cell and nuclear extracts using BARD1 polyclonal antibody (Product # PA5-85707) using 30 µg of sample at a dilution of 1:1000. Prior to incubation with primary antibody, the sample was separated on 7.5% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of BARD1 was performed by separating 30 µg of A431 whole cell and nuclear extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a BARD1 Polyclonal Antibody (Product # PA5-85707) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using BARD1 Polyclonal Antibody (Product # PA5-85707). A431 whole cell and nuclear extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with BARD1 Polyclonal Antibody (Product # PA5-85707) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- BARD1 Polyclonal Antibody detects BARD1 protein at nucleus by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BARD1 protein stained by BARD1 Polyclonal Antibody (Product # PA5-85707) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- BARD1 Polyclonal Antibody detects BARD1 protein at nucleus by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BARD1 protein stained by BARD1 Polyclonal Antibody (Product # PA5-85707) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- BARD1 Polyclonal Antibody detects BARD1 protein at nucleus in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. BARD1 Polyclonal Antibody (Product # PA5-85707) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- BARD1 Polyclonal Antibody detects BARD1 protein at nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. BARD1 Polyclonal Antibody (Product # PA5-85707) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- BARD1 Polyclonal Antibody detects BARD1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. BARD1 stained by BARD1 Polyclonal Antibody (Product # PA5-85707) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.