600-401-474

antibody from Invitrogen Antibodies

Targeting: PACRG FLJ32724, Glup, HAK005771, PARK2CRG

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

600-401-474

Provider

Invitrogen Antibodies

Product name

PACRG Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Chicken/Avian

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

1.6 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot using Rocklands Affinity Purified anti-PACRG antibody shows detection of a band ~40 kDa corresponding to human PACRG (arrowhead lane 1). Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide (lane 2). Approximately 35 µg of a mouse embryonic fibroblast (MEF) whole cell lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,500 for 2h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX (611-132-122) for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.