MAB4161

antibody from Novus Biologicals

Targeting: CSF1 M-CSF, MCSF, MGC31930

Western blot (Data presented on provider website)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB4161

Provider

Novus Biologicals

Product name

Rat Monoclonal M-CSF Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects mouse M-CSF in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human M-CSF or recombinant mouse SCF is observed.

Reactivity

Mouse

Host

Rat

Isotype

IgG

Vial size

500 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Bone-derived Nestin-positive mesenchymal stem cells improve cardiac function via recruiting cardiac endothelial cells after myocardial infarction.

Lu D, Liao Y, Zhu SH, Chen QC, Xie DM, Liao JJ, Feng X, Jiang MH, He W
Stem cell research & therapy 2019 Apr 27;10(1):127

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Interleukin-18 Amplifies Macrophage Polarization and Morphological Alteration, Leading to Excessive Angiogenesis.

Kobori T, Hamasaki S, Kitaura A, Yamazaki Y, Nishinaka T, Niwa A, Nakao S, Wake H, Mori S, Yoshino T, Nishibori M, Takahashi H
Frontiers in immunology 2018;9:334

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An inflammatory gene signature distinguishes neurofibroma Schwann cells and macrophages from cells in the normal peripheral nervous system.

Choi K, Komurov K, Fletcher JS, Jousma E, Cancelas JA, Wu J, Ratner N
Scientific reports 2017 Mar 3;7:43315

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CSF-1 receptor-mediated differentiation of a new type of monocytic cell with B cell-stimulating activity: its selective dependence on IL-34.

Yamane F, Nishikawa Y, Matsui K, Asakura M, Iwasaki E, Watanabe K, Tanimoto H, Sano H, Fujiwara Y, Stanley ER, Kanayama N, Mabbott NA, Magari M, Ohmori H
Journal of leukocyte biology 2014 Jan;95(1):19-31

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Colony-stimulating factor 1 receptor (CSF1R) signaling in injured neurons facilitates protection and survival.

Luo J, Elwood F, Britschgi M, Villeda S, Zhang H, Ding Z, Zhu L, Alabsi H, Getachew R, Narasimhan R, Wabl R, Fainberg N, James ML, Wong G, Relton J, Gambhir SS, Pollard JW, Wyss-Coray T
The Journal of experimental medicine 2013 Jan 14;210(1):157-72

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Osteoblasts support B-lymphocyte commitment and differentiation from hematopoietic stem cells.

Zhu J, Garrett R, Jung Y, Zhang Y, Kim N, Wang J, Joe GJ, Hexner E, Choi Y, Taichman RS, Emerson SG
Blood 2007 May 1;109(9):3706-12

Blocking/Neutralizing

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Cell Proliferation Induced by M-CSF and Neutralization by Mouse M-CSF Antibody. Recombinant Mouse M-CSF (Catalog # 416-ML) stimulates proliferation in the M-NFS-60 mouse myelogenous luekemia lymphoblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse M-CSF (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse M-CSF Monoclonal Antibody (Catalog # MAB4161). The ND₅₀ is typically 3-12 µg/mL.