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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- GTX81735 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX81735, RRID:AB_11173351
- Product name
- M-CSF antibody, Internal
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- Western blot analysis of M-CSF (arrow) using rabbit polyclonal M-CSF Antibody (Center) (GTX81735). 293 cell lysates (2 μg/lane) either nontransfected (Lane 1) or transiently transfected with the MCSF gene (Lane 2)
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of M-CSF Antibody (Center)(GTX81735) with MDA-MB231 cell followed by Alexa Fluor 488-conjμgated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- M-CSF Antibody (Center) (GTX81735)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjμgation of the secondary antibody and DAB staining.This data demonstrates the use of M-CSF Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- M-CSF Antibody (Center) (GTX81735) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjμgated goat-anti-rabbit secondary antibodies were used for the analysis.
