H00001435-M01

antibody from Abnova Corporation

Targeting: CSF1 M-CSF, MCSF, MGC31930

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00001435-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00001435-M01, RRID:AB_463869

Product name

CSF1 monoclonal antibody (M01), clone 1A9

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant CSF1.

Antigen sequence

EEVSEYCSHMIGSGHLQSLQRLIDSQMETSCQITF
EFVDQEQLKDPVCYLKKAFLLVQDIMEDTMRFRDN
TPNAIAIVQLQELSLRLKSCFTKDYEEHDK


Isotype

IgG

Antibody clone number

1A9

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

miR148b is a major coordinator of breast cancer progression in a relapse-associated microRNA signature by targeting ITGA5, ROCK1, PIK3CA, NRAS, and CSF1.

Cimino D, De Pittà C, Orso F, Zampini M, Casara S, Penna E, Quaglino E, Forni M, Damasco C, Pinatel E, Ponzone R, Romualdi C, Brisken C, De Bortoli M, Biglia N, Provero P, Lanfranchi G, Taverna D
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2013 Mar;27(3):1223-35

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Pathway-based biomarker search by high-throughput proteomics profiling of secretomes.

Lawlor K, Nazarian A, Lacomis L, Tempst P, Villanueva J
Journal of proteome research 2009 Mar;8(3):1489-503

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of CSF1 expression in transfected 293T cell line by CSF1 monoclonal antibody (M01), clone 1A9.Lane 1: CSF1 transfected lysate(60.1 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged CSF1 is approximately 3ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol