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Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- AB-216-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Human M-CSF Antibody
- Antibody type
- Polyclonal
- Description
- Protein A or G purified. Detects human M-CSF in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody shows less than 5% cross-reactivity with recombinant mouse M-CSF.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
NP_757350- Isotype
- IgG
- Vial size
- 1 mg
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Molecular signaling pathways mediating osteoclastogenesis induced by prostate cancer cells.
CD40-ligand stimulates myelopoiesis by regulating flt3-ligand and thrombopoietin production in bone marrow stromal cells.
Rafiei S, Komarova SV
BMC cancer 2013 Dec 26;13:605
BMC cancer 2013 Dec 26;13:605
CD40-ligand stimulates myelopoiesis by regulating flt3-ligand and thrombopoietin production in bone marrow stromal cells.
Solanilla A, Déchanet J, El Andaloussi A, Dupouy M, Godard F, Chabrol J, Charbord P, Reiffers J, Nurden AT, Weksler B, Moreau JF, Ripoche J
Blood 2000 Jun 15;95(12):3758-64
Blood 2000 Jun 15;95(12):3758-64
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Cell Proliferation Induced by M-CSF and Neutralization by Human M-CSF Antibody. Recombinant Human M-CSF (Catalog # 216-MC) stimulates proliferation in the M-NFS-60 mouse myelogenous leukemia lymphoblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human M-CSF (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human M-CSF Polyclonal Antibody (Catalog # AB-216-NA). The ND50 is typically 0.05-0.15 µg/mL.
