PA5-109257

antibody from Invitrogen Antibodies

Targeting: KCND3 KSHIVB, Kv4.3, SCA19, SCA22

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-109257

Provider

Invitrogen Antibodies

Product name

KV4.3 (KCND3) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Positive Control: MCF-7, mouse marrow tissue, LOVO, MG-63, human esophagus tissue, human placenta tissue, mouse cerebellum tissue.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles, store in dark

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of KV4.3 in Lane 1: MCF-7, Lane 2: Mouse marrow . Samples were incubated with KV4.3 polyclonal antibody (Product # PA5-109257), at a dilution of 1:1000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of KV4.3 in MG-63 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with KV4.3 polyclonal antibody (Product # PA5-109257), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of KV4.3 in MCF-7 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with KV4.3 polyclonal antibody (Product # PA5-109257), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of KV4.3 in LOVO cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with KV4.3 polyclonal antibody (Product # PA5-109257), followed by DAPI (blue).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of KV4.3 in paraffin-embedded mouse cerebellum tissue. Samples were incubated with KV4.3 polyclonal antibody (Product # PA5-109257), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of KV4.3 in paraffin-embedded mouse cerebellum tissue. Samples were incubated with KV4.3 polyclonal antibody (Product # PA5-109257), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of KV4.3 in paraffin-embedded human placenta tissue. Samples were incubated with KV4.3 polyclonal antibody (Product # PA5-109257), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of KV4.3 in paraffin-embedded human esophagus tissue. Samples were incubated with KV4.3 polyclonal antibody (Product # PA5-109257), and followed by hematoxylin.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of KV4.3 in MG-63 cells (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Samples were incubated with KV4.3 polyclonal antibody (Product # PA5-109257) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.