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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [3]
- Immunohistochemistry [3]
- Flow cytometry [1]
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- Product number
- MA5-42480 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- WNT2B Recombinant Rabbit Monoclonal Antibody (JB48-34)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: SiHa, Hela, LOVO, SHSY-5Y, human kidney tissue, mouse kidney tissue, rat testis tissue.
- Antibody clone number
- JB48-34
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Wnt2b on SiHa cell lysates using WNT2B Recombinant Monoclonal Antibody (Product # MA5-42480).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of Wnt2b in Hela cells (green) using WNT2B Recombinant Monoclonal Antibody (Product # MA5-42480). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of Wnt2b in LOVO cells (green) using WNT2B Recombinant Monoclonal Antibody (Product # MA5-42480). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of Wnt2b in SHSY-5Y cells (green) using WNT2B Recombinant Monoclonal Antibody (Product # MA5-42480). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of paraffin-embedded rat testis tissue using WNT2B Recombinant Monoclonal Antibody (Product # MA5-42480). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of paraffin-embedded human kidney tissue using WNT2B Recombinant Monoclonal Antibody (Product # MA5-42480). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of paraffin-embedded mouse kidney tissue using WNT2B Recombinant Monoclonal Antibody (Product # MA5-42480). Counter stained with hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of WNT2B in LOVO cells using WNT2B Recombinant Monoclonal Antibody (Product # MA5-42480) at 1:50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
