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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
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- Product number
- NB100-77314 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-77314, RRID:AB_1085095
- Product name
- Rabbit Polyclonal MOV10 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Mov10 and APOBEC3G localization to processing bodies is not required for virion incorporation and antiviral activity.
The mammalian TRIM-NHL protein TRIM71/LIN-41 is a repressor of mRNA function.
Izumi T, Burdick R, Shigemi M, Plisov S, Hu WS, Pathak VK
Journal of virology 2013 Oct;87(20):11047-62
Journal of virology 2013 Oct;87(20):11047-62
The mammalian TRIM-NHL protein TRIM71/LIN-41 is a repressor of mRNA function.
Loedige I, Gaidatzis D, Sack R, Meister G, Filipowicz W
Nucleic acids research 2013 Jan 7;41(1):518-32
Nucleic acids research 2013 Jan 7;41(1):518-32
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Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MOV10 Antibody [NB100-77314] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-MOV10 antibody used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. MOV10 was also immunoprecipitated by a previous lot (NB100-77314) of this antibody. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).
